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:: Volume 22, Number 3 (Scientific Journal of Kurdistan University of Medical Sciences 2017) ::
SJKU 2017, 22(3): 60-73 Back to browse issues page
The protective effect of aerobic exercise on breast cancer by TGFβ protein and Smad-3 and MMP2 gene in female mice
Vahid Moghadam1, Dr Maghsood Piri 2, Dr Mohammad Ali Azarbayjani3, Dr Hasan Matin Homaee4
1- Department of Exercise Physiology, Islamic Azad University Central Tehran Branch, Tehran, Iran
2- Professor Department of Exercise Physiology, Islamic Azad University Central Tehran Branch, Tehran, Iran , m.peeri@iauctb.ac.ir
3- Professor Department of Exercise Physiology, Islamic Azad University Central Tehran Branch, Tehran, Iran
4- Associate Professor Department of Exercise Physiology, Islamic Azad University Central Tehran Branch, Tehran, Iran
Abstract:  
Background and Aim: The aim of this study was to investigate the protective effect of aerobic exercise on breast cancer by TGFβ protein and Smad-3 and MMP2 gene in female mice.
Material and method: Twenty-four female Balb/c mice were divided into control and exercise groups. Estrogen-receptor dependent breast cancer cells (MC4-L2) were injected into the subcutaneous area of the right flank of the mice. the exercise group performed endurance exercise five sessions per week at an intensity of 60% to 65% VO2max for 10 weeks. Each session lasted 60 minutes. After appearance of the tumor, the length and width of the tumor were measured by a digital caliper once a week. Finally, the mice were sacrificed, tumor tissue was removed and immediately frozen and kept at -70° C. The level of TGFβ protein was measured by western blot and expression of Smad and mmp2 genes in the samples was measured by Real Time PCR. Data were analyzed by t-test. P<0.05 was considered significant.
Results: The results showed that aerobic continuous training significantly increased mean expression of Smad-3 (p=0/033) and TGF-β protein mean concentration (P=0/000) in tumor tissue in the endurance group compared to those in the control group. Also, the results showed that aerobic training significantly decreased the mean expression of MMP-2 in tumor tissue in the endurance group compared to that in the control group (p=0/044).
Conclusion: According to the results, It seems that continuous aerobic training may be an effective intervention to reduce breast cancer progression.
Key words: Breast cancer, Aerobic continuous training, TGFβ, Smad, MMP2.
 
Received: Jul 11, 2016      Accepted: Apr 16, 2017
Keywords: Breast cancer, Aerobic continuous training, TGFβ, Smad, MMP2.
Full-Text [PDF 480 kb]      
Type of Study: Research | Subject: General
Received: 2017/07/24 | Accepted: 2017/07/24 | Published: 2017/07/24
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Volume 22, Number 3 (Scientific Journal of Kurdistan University of Medical Sciences 2017) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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