:: Volume 22, Number 3 (Scientific Journal of Kurdistan University of Medical Sciences 2017) ::
SJKU 2017, 22(3): 49-59 Back to browse issues page
Effects of taurine and homocysteine on lipid profile and oxidative stress in fructose-fed rats
Dr Ali Akbar Abolfathi1, Dr Zakarya Vahabzadeh 2, Dr Naser Mahmoodiaghdam3, Davood Vahabzadeh4, Dr Mohammad Saeed Hakhamanesh5
1- Associate Professor Department of Clinical Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
2- Assistant Professor, Liver & Digestive Research Centre, Kurdistan University of Medical Sciences, Sanandaj, Iran. , zakariav@yahoo.com
3- Veterinary School of Medicine, Urmia University, Urmia, Iran
4- Maternal and Childhood Obesity Research Centre, Urmia University of Medical Sciences, Urmia, Iran.
5- Associate Professor Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Abstract:  
Background and Aim: Consumption of large doses of fructose have been associated with numerous metabolic abnormalities in human beings and laboratory animals. Homocysteine is believed to enhance oxidative stress while taurine has hypolipidemic effect. The present study was performed to investigate the effects of taurine and homocysteine on the oxidative stress and lipid profile in rats receiving a high fructose-containing diet.
Material and Method: In this experimental study, forty male adult Wistar rats were divided in to 5 groups. Our control group (group 1) received a normal chow.  The diets of the second to fifth groups were as following:
Group 2: A high fructose containing diet
Group 3: A high fructose containing diet + homocysteine thiolactate (50mg/kg /day)
Group 4: A high fructose containing diet + taurine (2%)
Groups 5: A high fructose containing diet + homocysteine thiolactate (50mg/kg /day)+ taurine (2%)
All the groups received the above mentioned diet for six-weeks. At the end of the experimental period, total antioxidant capacity, triglycerides, HDL, LDL, and total cholesterol of the plasma were measured. Activities of glutathione peroxidase and superoxide dismutase and level of malondialdehyde were measured in the heart and kidney tissues of the rats.
Result: Fructose alone or in addition to homocysteine significantly reduced total antioxidant capacity, glutathione peroxidase, and superoxide dismutase activities, while the levels of triglycerides and malondialdehyde increased. Taurine suppressed or attenuated these changes.
Conclusion: The results of this study confirmed the role of high fructose containing diet in inducing oxidative stress and hypertriglyceridemia. We also found that use of homocysteine deteriorated the effects of high fructose containing diet. In contrast taurine had beneficial effects and suppressed fructose induced-oxidative stress and hypertriglyceridemia.
Keywords: Fructose, Homocysteine, Taurine, Oxidative Stress, Rat.
 
Received: Dec 5, 2016      Accepted: Jan 22, 2017
Keywords: Fructose, Homocysteine, Taurine, Oxidative Stress, Rat.
Full-Text [PDF 329 kb]      
Type of Study: Research | Subject: General
Received: 2017/07/24 | Accepted: 2017/07/24 | Published: 2017/07/24



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Volume 22, Number 3 (Scientific Journal of Kurdistan University of Medical Sciences 2017) Back to browse issues page