:: Volume 22, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2017) ::
SJKU 2017, 22(2): 120-128 Back to browse issues page
Isolation, morphological characterization and host range determination of Iranian bacteriophages against Salmonella reference collection
Pegah Panahi , Ali Mojtahedi Dr * 1, Mohammad Ali Khan Mirzaei Dr, Mohammad Shenagari Dr2, Zahra Atrkar Roushan Dr2
1- Associate Professor , alimojtahedi@yahoo.com
2- Assistant Professor
Abstract:   (1236 Views)

Background and Aim: Salmonella spp. is important in medicine. Most related infections are self-limited. Antibiotics are used for high risk patients such as children, old individuals and immune-compromised patients. Overuse of antibiotics has led to increased emergence of multi-drug resistant bacteria which are life-threatening. Phage therapy is an alternative method to reduce antibiotic use.

Materials and Methods: Waste water samples were collected from sewage system of Rasht City twice a month. Prepared suspensions of Salmonella reference collection A (SARA) number 1, 6 (S. typhimurium) and 46, 48, 51 (S. paratyphi B) and double LB broth were mixed with filtrated waste water. After centrifugation and filtration, serial dilutions were prepared and phages were isolated. Morphologic characteristics were determined using TEM (Transmission electron microscopy). Finally, to assess the spectrum effect of the bacteriophages, we determined host range against 19 SARA strains.

Results: Clear plaque formation on double layer LB agar indicated lysis of the test strains by isolated phages.  The results of host range showed that some of the phages were able to lyse a number of other bacteria of SARA collection. Imaging with TEM indicated that the isolated phages against SARA collection belonged to Siphoviridae and Podoviridae families.

Conclusion: This is the first report of phage isolation against Salmonella reference collection in Iran. SARA no. 51 (S. paratyphi B) was lysed by S. typhimurium phage. It means that the isolated phage may lyse S. typhi, which is an important human pathogen.

Keywords: Salmonella reference collection, Bacteriophage, TEM, Morphology.

Received: Oct 31, 2016      Accepted: Jan 10, 2017

Keywords: Salmonella reference collection, Bacteriophage, TEM, Morphology.
Full-Text [PDF 3282 kb]   (514 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/05/28 | Accepted: 2017/05/28 | Published: 2017/05/28

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Volume 22, Issue 2 (Scientific Journal of Kurdistan University of Medical Sciences 2017) Back to browse issues page