:: Volume 22, Number 1 (Scintific Journal of Kurdistan University of Medical Sciences 2017) ::
SJKU 2017, 22(1): 93-106 Back to browse issues page
Evaluation of the protective effect of curcumin on liver tissue in NMRI mice treated with sodium arsenite
Dr Seyed Mohammad Ali Shariatzadeh 1, Dr Malek Soleimani-Mehranjani2, Samira Naderi-Noreini3
1- Professor Department of Biology, Faculty of Science, Arak University, Arak, Iran , s-shariatzadeh@araku.ac.ir
2- Professor Department of Biology, Faculty of Science, Arak University, Arak, Iran
3- Department of Biology, Faculty of Science, Arak University, Arak, Iran
Abstract:  

Background and Aim: Arsenic (As) compounds are environmental toxicants which are among human carcinogens. Sodium arsenite exposure leads to its accumulation in the liver resulting in liver disorders. The aim of this study was to investigate the protective effect of curcumin, as an antioxidant, on the liver tissue in the mice exposed to sodium arsenite.

Material and Methods: Thirty NMRI mice with mean body weight of 31±2 g. were randomly divided into 5 groups: control, scheme (receiving DMSO),curcumin (15mg/kg/day), sodium arsenite (5mg/kg/day) and sodium arsenite+curcumin groups. Every group consisted of 6 mice. The exposure was by intraperitoneal injections and carried out for 5 weeks. Then the mice were killed and the liver tissue was removed and weighed. Histopathological and stereological analyses were performed and the incidence of hepatocyte cells apoptosis (by the TUNEL method) was determined. Data were analyzed using one way ANOVA, and the differences among mean values were considered significant at P<0.05.  

Results: A significant increase in the mean relative weight of liver, total volume of sinusoids, bile ductules (p<0.001) and total number of hepatocytes (p<0.03) and a significant decrease in the total volume of the central veins (p<0.001), the mean volume of the hepatocytes (p<0.04) and their nuclei (p<0.001) were observed in sodium arsenite group compared to those in control and scheme groups. Histopathological examination also revealed parenchymal disorganization, inflammatory cell infiltration, necrosis of hepatocytes and destruction of reticulin fiber scaffold in the mice liver treated with sodium arsenite. Most of sodium arsenite-induced liver damage improved in the sodium arsenite + curcumin group to the same extent as control group (p<0.05).

Conclusion:  Treatment with curcumin reduced liver damage induced by sodium arsenite.

Keywords: Curcumin, Liver, Mouse, Sodium arsenite, Stereology.

 

Received: Oct 31, 2015      Accepted: Dec 21, 2016

Keywords: Curcumin, Liver, Mouse, Sodium arsenite, Stereology
Full-Text [PDF 5091 kb]      
Type of Study: Research | Subject: General
Received: 2017/03/14 | Accepted: 2017/03/14 | Published: 2017/03/14



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Volume 22, Number 1 (Scintific Journal of Kurdistan University of Medical Sciences 2017) Back to browse issues page