[Home ] [Archive]   [ فارسی ]  
:: Volume 19, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2014) ::
SJKU 2014, 19(3): 94-102 Back to browse issues page
Compression of verification of neonatal mouse ovaries using two carriers: straw and cryolock
Shabnam Abdi 1, Mozhdeh Salehnia Dr * 2, Saman Hosseinkhani 3
1- Tarbiat Modares University
2- Professor Tarbiat Modares University
3- Associate Professor Tarbiat Modares University
Abstract:   (2916 Views)
Background and Aim: Vitrification of ovarian tissue is an effective method for preservation of fertility. Many attempts have been made to improve cryopreservation conditions. The aim of this study was to compare the effects of two vitrification methods using two carrier systems straw and cryolock, on the morphology of neonatal mouse ovarian tissue. Materials and Method: In this experimental study we used NMRI 7-day-old mice. Their ovaries were excised and divided into non-vitrified, vitrified with straw, vitrified with cryolock and toxicity test groups. In vitrification group, the ovaries were dehydrated in a mixture of 40% ethylene glycol, 30% ficoll 70 and sucrose (EGFS40) for 5 min. Then they were put in a plastic straw or cryolock and were placed into liquid nitrogen and stored for one week. Whereas in the toxicity test group the ovaries were exposed to the cryoprotectant EGFS40 and warming solution procedure without using liquid nitrogen. Then we studied the morphology of ovarian follicles by means of light and electron microscopes. Result: No significant differences were observed between the groups in regard to the percentages of primordial and primary follicles with normal morphology. But the percentages of normal preantral follicles in the vitrified group using straw were significantly lower than those of other groups. Ultrastructural studies showed signs of degeneration and nuclear shrinkage in follicular cells in some of the preantral follicles in vitrified group using straw. Conclusion: Vitrification of ovarian tissue using cryolock is more effective for preserveration of the normal morphology of ovarian tissue, compared to vitrification by using straw. Keywords: Vitrification, Ovarian tissue, Straw, Cryolock. Received: May 21, 2013 Accepted: May 28, 2014
Keywords: Vitrification, Ovarian tissue, Straw, Cryolock.
Full-Text [PDF 2495 kb]   (692 Downloads)    
Type of Study: Research | Subject: General
Received: 2014/11/9 | Accepted: 2014/11/9 | Published: 2014/11/9
Add your comments about this article
Your username or Email:

CAPTCHA code



XML   Persian Abstract   Print



Volume 19, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2014) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.11 seconds with 30 queries by YEKTAWEB 3731