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Generation of induced pluripotent stem cells from mesenchymal stem cells of human umbilical cord vein
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Mehdad Abdi1   , Sona Zare1 , Fardin Fathi 2, Seyed Hadi Anjamrooz1 , Jafar Rezaei1 , Jalal Rostamzadeh1 |
1- Kurdistan University of Medical Sciences
2- Kurdistan University of Medical Sciences , farfath@gmail.com |
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Abstract: (10404 Views) |
| Background and Aim: The aim of this study was to generate induced pluripotent stem cells from human umbilical cord vein mesenchymal stem cells by use of plasmid vector.
Material and Methods: In this experimental study using type IV collagenase enzyme, we extracted and cultured mesenchymal stem cells obtained from human umbilical cord vein wall.Usingelectroporation method, these cells were transfected with plasmid vector which carried selfrenewal transcription factors of OCT4 and SOX2.After 9 days we observed the induced pluripotent stem cells like colonies. The nature of thesecells were evaluated byimmunocytochemistry and alkalin phosphatase activity of embryonic stem cells.
Results: Immunocytochemical analysis showed that these cells express the pluripotency markerssuch as OCT4, SSEA4, TRA1-60, TRA1-81, and also hadalkalin Phosphatase activity.
Conclusion: This study revealed that transient expression of self renewal genes of OCT4 and SOX2 couldlead todevelopment of induced pluripotent stem cells like colonies from umbilical cord vein mesenchymal stem cells.
Received: May 13, 2013 Accepted: Aug 25, 2013
Conflict of interest: None declared |
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| Keywords: Embryonic stem cells, Umbilical cord vein mesenchymal stem cells, Reprogramming, Selfrenewal genes. |
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Full-Text [PDF 342 kb]
(4185 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2014/03/16 | Accepted: 2014/03/16 | Published: 2014/03/16
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